gzmb cre Search Results


gzmb ki-cre  (inGenious Targeting Laboratory)
90
inGenious Targeting Laboratory gzmb ki-cre
A) UMAP projection of CD45⁺CD103⁺ IEL isolated from the small intestinal epithelium, revealing phenotypic heterogeneity across subsets. Accompanying pie chart depicts relative frequencies of each subset. Representative of three mice. B) Area-proportional Venn diagram illustrating shared proteomic profiles among three major IEL subsets: TCRαβ⁺CD8αα⁺, TCRγδ⁺CD8αα⁺, and TCRαβ⁺CD8αβ⁺. C) UMAP embedding of CD45⁺CD3⁺CD8α⁺ IEL profiled by single-cell ATAC-seq, demonstrating convergence in chromatin accessibility landscapes. D–E) Representative intracellular flow cytometry histograms of <t>GzmB</t> protein expression in (D) IEL and (E) peripheral immune populations, including NK cells, thymocytes, and splenic T cells. F) Confocal images of jejunal tissue sections stained for GzmB (green), CD3 (red), E-cadherin (gray), and nuclei (DAPI, blue), showing GzmB⁺CD3⁺ cells localized to the epithelial compartment. Scale bar, 100 μm. Representative of five mice. G) Schematic of the Gzmb -Cre knock-in allele, in which a T2A-Cre cassette is inserted downstream of the endogenous Gzmb stop codon, enabling bicistronic expression. H) Pie chart depicting the relative frequencies of each IEL subset in Gzmb Cre/+ mice. Representative of three mice. I) Representative immunofluorescence image of jejunal sections from Gzmb Cre/+ × Rosa26 LsL-tdTomato mice, showing tdTomato⁺ IELs (red) within the epithelium. Actin (phalloidin, green); nuclei (DAPI, blue). Representative of three mice. J) Flow cytometry plots and pooled quantification of tdTomato⁺ frequencies across IEL subsets, with individual bar labels denoting mean percentage of tdTomato⁺ cells within each population. Data represents mean ± SEM from 3 mice/group.
Gzmb Ki Cre, supplied by inGenious Targeting Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gzmb+cre/gzmb+ki+cre/bio_rxiv__2025__05__14__654011-162-0-5
Average 90 stars, based on 1 article reviews
gzmb ki-cre - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


A) UMAP projection of CD45⁺CD103⁺ IEL isolated from the small intestinal epithelium, revealing phenotypic heterogeneity across subsets. Accompanying pie chart depicts relative frequencies of each subset. Representative of three mice. B) Area-proportional Venn diagram illustrating shared proteomic profiles among three major IEL subsets: TCRαβ⁺CD8αα⁺, TCRγδ⁺CD8αα⁺, and TCRαβ⁺CD8αβ⁺. C) UMAP embedding of CD45⁺CD3⁺CD8α⁺ IEL profiled by single-cell ATAC-seq, demonstrating convergence in chromatin accessibility landscapes. D–E) Representative intracellular flow cytometry histograms of GzmB protein expression in (D) IEL and (E) peripheral immune populations, including NK cells, thymocytes, and splenic T cells. F) Confocal images of jejunal tissue sections stained for GzmB (green), CD3 (red), E-cadherin (gray), and nuclei (DAPI, blue), showing GzmB⁺CD3⁺ cells localized to the epithelial compartment. Scale bar, 100 μm. Representative of five mice. G) Schematic of the Gzmb -Cre knock-in allele, in which a T2A-Cre cassette is inserted downstream of the endogenous Gzmb stop codon, enabling bicistronic expression. H) Pie chart depicting the relative frequencies of each IEL subset in Gzmb Cre/+ mice. Representative of three mice. I) Representative immunofluorescence image of jejunal sections from Gzmb Cre/+ × Rosa26 LsL-tdTomato mice, showing tdTomato⁺ IELs (red) within the epithelium. Actin (phalloidin, green); nuclei (DAPI, blue). Representative of three mice. J) Flow cytometry plots and pooled quantification of tdTomato⁺ frequencies across IEL subsets, with individual bar labels denoting mean percentage of tdTomato⁺ cells within each population. Data represents mean ± SEM from 3 mice/group.

Journal: bioRxiv

Article Title: Intraepithelial lymphocytes exhibit selective immunity to intestinal pathogens

doi: 10.1101/2025.05.14.654011

Figure Lengend Snippet: A) UMAP projection of CD45⁺CD103⁺ IEL isolated from the small intestinal epithelium, revealing phenotypic heterogeneity across subsets. Accompanying pie chart depicts relative frequencies of each subset. Representative of three mice. B) Area-proportional Venn diagram illustrating shared proteomic profiles among three major IEL subsets: TCRαβ⁺CD8αα⁺, TCRγδ⁺CD8αα⁺, and TCRαβ⁺CD8αβ⁺. C) UMAP embedding of CD45⁺CD3⁺CD8α⁺ IEL profiled by single-cell ATAC-seq, demonstrating convergence in chromatin accessibility landscapes. D–E) Representative intracellular flow cytometry histograms of GzmB protein expression in (D) IEL and (E) peripheral immune populations, including NK cells, thymocytes, and splenic T cells. F) Confocal images of jejunal tissue sections stained for GzmB (green), CD3 (red), E-cadherin (gray), and nuclei (DAPI, blue), showing GzmB⁺CD3⁺ cells localized to the epithelial compartment. Scale bar, 100 μm. Representative of five mice. G) Schematic of the Gzmb -Cre knock-in allele, in which a T2A-Cre cassette is inserted downstream of the endogenous Gzmb stop codon, enabling bicistronic expression. H) Pie chart depicting the relative frequencies of each IEL subset in Gzmb Cre/+ mice. Representative of three mice. I) Representative immunofluorescence image of jejunal sections from Gzmb Cre/+ × Rosa26 LsL-tdTomato mice, showing tdTomato⁺ IELs (red) within the epithelium. Actin (phalloidin, green); nuclei (DAPI, blue). Representative of three mice. J) Flow cytometry plots and pooled quantification of tdTomato⁺ frequencies across IEL subsets, with individual bar labels denoting mean percentage of tdTomato⁺ cells within each population. Data represents mean ± SEM from 3 mice/group.

Article Snippet: Gzmb KI-Cre was generated by Ingenious Targeting Laboratory for Dr. Mahima Swamy.

Techniques: Isolation, Flow Cytometry, Expressing, Staining, Knock-In, Immunofluorescence

A ) Flow cytometry gating strategy used to define IEL subsets from small intestinal epithelial preparations. B) Sorting strategy for scATAC-seq analysis. Live CD45⁺CD3⁺CD8α⁺ IEL were isolated for chromatin profiling. C–D) Bar graphs quantifying the proportion of IELs with accessible chromatin at (C) Itgae (encoding CD103) and (D) Ifng , canonical markers of IEL identity and effector function. E) Area-proportional Venn diagram illustrating the overlap in accessible chromatin regions between TCRγδ⁺ and TCRαβ⁺ IEL based on previously published bulk ATAC-seq data (Semenkovich et al., 2016), indicating extensive epigenetic convergence. F) Normalized RNA expression of Gzma and Gzmb across T cell developmental stages, mature T cell subsets, and NK cells, based on publicly available ImmGen datasets ( https://www.immgen.org/ ). G) Representative histogram showing inefficient IEL labeling in previously reported GzmbCre transgenic mice crossed to Rosa26 lsl-EYFP . H) Representative histogram of tdTomato⁺ IEL in Gzmb Cre/+ × Rosa26 lsl-tdTomato mice, demonstrating robust and specific labeling across IEL subsets. I) Flow cytometry quantification of intracellular GzmB protein expression across major IEL subsets from Gzmb Cre/+ mice.

Journal: bioRxiv

Article Title: Intraepithelial lymphocytes exhibit selective immunity to intestinal pathogens

doi: 10.1101/2025.05.14.654011

Figure Lengend Snippet: A ) Flow cytometry gating strategy used to define IEL subsets from small intestinal epithelial preparations. B) Sorting strategy for scATAC-seq analysis. Live CD45⁺CD3⁺CD8α⁺ IEL were isolated for chromatin profiling. C–D) Bar graphs quantifying the proportion of IELs with accessible chromatin at (C) Itgae (encoding CD103) and (D) Ifng , canonical markers of IEL identity and effector function. E) Area-proportional Venn diagram illustrating the overlap in accessible chromatin regions between TCRγδ⁺ and TCRαβ⁺ IEL based on previously published bulk ATAC-seq data (Semenkovich et al., 2016), indicating extensive epigenetic convergence. F) Normalized RNA expression of Gzma and Gzmb across T cell developmental stages, mature T cell subsets, and NK cells, based on publicly available ImmGen datasets ( https://www.immgen.org/ ). G) Representative histogram showing inefficient IEL labeling in previously reported GzmbCre transgenic mice crossed to Rosa26 lsl-EYFP . H) Representative histogram of tdTomato⁺ IEL in Gzmb Cre/+ × Rosa26 lsl-tdTomato mice, demonstrating robust and specific labeling across IEL subsets. I) Flow cytometry quantification of intracellular GzmB protein expression across major IEL subsets from Gzmb Cre/+ mice.

Article Snippet: Gzmb KI-Cre was generated by Ingenious Targeting Laboratory for Dr. Mahima Swamy.

Techniques: Flow Cytometry, Isolation, RNA Expression, Labeling, Transgenic Assay, Expressing

A) Representative immunofluorescence image of jejunal tissue from IEL iDTR ( G zmb Cre/+ × Rosa26 LsL-iDTR ) and control IEL WT (Gzmb Cre/+ ) mice 24 hours following a single intraperitoneal injection of diphtheria toxin (DT, 50 μg/kg), showing loss of CD3⁺ IELs in the epithelium. Scale bar, 100 μm. Representative of 5 mice. B) Flow cytometric quantification of CD45⁺CD103⁺ IELs and CD45⁺CD103⁻ cells in the epithelial fraction 24 h post-DT. Data represents mean ± SEM from 3 mice/group. C) Quantification of TCRγδ⁺ and TCRβ⁺ cells by flow cytometry in both IEL and lamina propria (gated on CD45+CD103-) (LP) compartments of the small intestine 24 h post-DT. Data represents mean ± SEM from 3 mice/group. D) Percent decrease across individual IEL subsets in IEL iDTR mice 24 h after DT, compared to IEL WT with individual bar labels denoting mean percentage of cells deleted within each population. Data represents mean ± SEM from 3 mice/group. E) Flow cytometric analysis of TCRγδ⁺ and TCRβ⁺ T cell populations in the spleen 24 h post-DT administration. Data represents mean ± SEM from 3 mice/group. F–G) Time-course quantification of total IELs (CD45⁺CD103⁺), TCRγδ⁺, and TCRαβ⁺ subsets at multiple time points post-DT (days 1, 4, 7, 10, 15), showing near-complete repopulation by day 15. Data represents mean ± SEM from 3 mice/group. H) Distribution of IEL subsets 15 days following DT treatment, demonstrating restoration of pre-depletion composition. Representation of 3 mice/group. I–J) Quantification of IEL and spleen T cells recovery at day 7 post-DT in mice treated with FTY720 (to block thymic egress) versus vehicle, demonstrating thymus-independent regeneration. Data represents mean ± SEM from 5 mice/group. K) EdU incorporation in CD45⁺CD103⁺ IEL on day 4 post-DT, indicating robust local proliferation during repopulation. Data represents mean ± SEM from 5 mice/group. L–M) Ki67 expression in IEL across time points post-depletion, showing a transient spike in proliferation that returns to baseline by day 15. Data represents mean ± SEM from 4-6 mice/group. Statistical analyses: ANOVA with Sidak’s post hoc correction (B,C,E, F, G, L, M); unpaired t-test (I, J, K). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns = non-significant.

Journal: bioRxiv

Article Title: Intraepithelial lymphocytes exhibit selective immunity to intestinal pathogens

doi: 10.1101/2025.05.14.654011

Figure Lengend Snippet: A) Representative immunofluorescence image of jejunal tissue from IEL iDTR ( G zmb Cre/+ × Rosa26 LsL-iDTR ) and control IEL WT (Gzmb Cre/+ ) mice 24 hours following a single intraperitoneal injection of diphtheria toxin (DT, 50 μg/kg), showing loss of CD3⁺ IELs in the epithelium. Scale bar, 100 μm. Representative of 5 mice. B) Flow cytometric quantification of CD45⁺CD103⁺ IELs and CD45⁺CD103⁻ cells in the epithelial fraction 24 h post-DT. Data represents mean ± SEM from 3 mice/group. C) Quantification of TCRγδ⁺ and TCRβ⁺ cells by flow cytometry in both IEL and lamina propria (gated on CD45+CD103-) (LP) compartments of the small intestine 24 h post-DT. Data represents mean ± SEM from 3 mice/group. D) Percent decrease across individual IEL subsets in IEL iDTR mice 24 h after DT, compared to IEL WT with individual bar labels denoting mean percentage of cells deleted within each population. Data represents mean ± SEM from 3 mice/group. E) Flow cytometric analysis of TCRγδ⁺ and TCRβ⁺ T cell populations in the spleen 24 h post-DT administration. Data represents mean ± SEM from 3 mice/group. F–G) Time-course quantification of total IELs (CD45⁺CD103⁺), TCRγδ⁺, and TCRαβ⁺ subsets at multiple time points post-DT (days 1, 4, 7, 10, 15), showing near-complete repopulation by day 15. Data represents mean ± SEM from 3 mice/group. H) Distribution of IEL subsets 15 days following DT treatment, demonstrating restoration of pre-depletion composition. Representation of 3 mice/group. I–J) Quantification of IEL and spleen T cells recovery at day 7 post-DT in mice treated with FTY720 (to block thymic egress) versus vehicle, demonstrating thymus-independent regeneration. Data represents mean ± SEM from 5 mice/group. K) EdU incorporation in CD45⁺CD103⁺ IEL on day 4 post-DT, indicating robust local proliferation during repopulation. Data represents mean ± SEM from 5 mice/group. L–M) Ki67 expression in IEL across time points post-depletion, showing a transient spike in proliferation that returns to baseline by day 15. Data represents mean ± SEM from 4-6 mice/group. Statistical analyses: ANOVA with Sidak’s post hoc correction (B,C,E, F, G, L, M); unpaired t-test (I, J, K). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns = non-significant.

Article Snippet: Gzmb KI-Cre was generated by Ingenious Targeting Laboratory for Dr. Mahima Swamy.

Techniques: Immunofluorescence, Control, Injection, Flow Cytometry, Blocking Assay, Expressing

A ) Flow cytometric quantification of CD45⁺, CD45⁺CD103⁺ (IEL), and CD45⁺CD103⁻ (non-IEL) subsets in the small intestine of Gzmb Cre/+ mice 24 hours after diphtheria toxin (DT) or PBS administration. Data represent mean ± SEM from 3 mice per group. B) Quantification of individual IEL subsets in the small intestine of IEL WT and IEL iDTR mice at day 1 post-DT, confirming broad depletion across γδ and αβ IEL lineages. Data represent mean ± SEM from 3 mice per group. C) Flow cytometric analysis of total CD45⁺ immune cells in multiple peripheral and mucosal compartments, including Peyer’s patches, colon IEL, colon lamina propria (LP), mesenteric lymph nodes (mLN), liver, and thymus of IEL WT and IEL iDTR mice, 24 hours after DT treatment. Data represent mean ± SEM from 3 mice per group. D–G) Quantification of TCRγδ⁺ and TCRβ⁺ lymphocyte subsets across mucosal and systemic tissues—Peyer’s patches, colon IEL, colon LP, mLN, and liver of IEL WT and IEL iDTR mice —24 hours post-DT administration. Data represent mean ± SEM from 3 mice per group. Statistical comparisons: ANOVA with Sidak’s post hoc correction (A, D, E, F, G) and unpaired t-test (B, C). * p < 0.05, ** p < 0.01, *** p < 0.001, ns = non-significant.

Journal: bioRxiv

Article Title: Intraepithelial lymphocytes exhibit selective immunity to intestinal pathogens

doi: 10.1101/2025.05.14.654011

Figure Lengend Snippet: A ) Flow cytometric quantification of CD45⁺, CD45⁺CD103⁺ (IEL), and CD45⁺CD103⁻ (non-IEL) subsets in the small intestine of Gzmb Cre/+ mice 24 hours after diphtheria toxin (DT) or PBS administration. Data represent mean ± SEM from 3 mice per group. B) Quantification of individual IEL subsets in the small intestine of IEL WT and IEL iDTR mice at day 1 post-DT, confirming broad depletion across γδ and αβ IEL lineages. Data represent mean ± SEM from 3 mice per group. C) Flow cytometric analysis of total CD45⁺ immune cells in multiple peripheral and mucosal compartments, including Peyer’s patches, colon IEL, colon lamina propria (LP), mesenteric lymph nodes (mLN), liver, and thymus of IEL WT and IEL iDTR mice, 24 hours after DT treatment. Data represent mean ± SEM from 3 mice per group. D–G) Quantification of TCRγδ⁺ and TCRβ⁺ lymphocyte subsets across mucosal and systemic tissues—Peyer’s patches, colon IEL, colon LP, mLN, and liver of IEL WT and IEL iDTR mice —24 hours post-DT administration. Data represent mean ± SEM from 3 mice per group. Statistical comparisons: ANOVA with Sidak’s post hoc correction (A, D, E, F, G) and unpaired t-test (B, C). * p < 0.05, ** p < 0.01, *** p < 0.001, ns = non-significant.

Article Snippet: Gzmb KI-Cre was generated by Ingenious Targeting Laboratory for Dr. Mahima Swamy.

Techniques:

A–B ) Time-course quantification of (A) TCRγδ⁺ and (B) TCRβ⁺ IEL subsets following a single DT injection in IEL WT and IEL iDTR mice, demonstrating progressive repopulation over 15 days. Data represent mean ± SEM from 3-5 mice per group. C–D) Representative histograms of tdTomato expression in (D) CD45⁺CD103⁺ and (E) TCRγδ⁺ and TCRβ⁺ IEL from Gzmb CreERT2 × Rosa26 lsl-tdTomato mice, assessed 7 days after final tamoxifen or oil-only control administration. E) Quantification of tdTomato⁺ IEL across indicated time points, demonstrating the longevity of labelled IEL subsets. Data represent mean ± SEM from 4 mice per group. Statistical comparisons: ANOVA with Sidak’s post hoc correction (A, B). * p < 0.05, ** p < 0.01.

Journal: bioRxiv

Article Title: Intraepithelial lymphocytes exhibit selective immunity to intestinal pathogens

doi: 10.1101/2025.05.14.654011

Figure Lengend Snippet: A–B ) Time-course quantification of (A) TCRγδ⁺ and (B) TCRβ⁺ IEL subsets following a single DT injection in IEL WT and IEL iDTR mice, demonstrating progressive repopulation over 15 days. Data represent mean ± SEM from 3-5 mice per group. C–D) Representative histograms of tdTomato expression in (D) CD45⁺CD103⁺ and (E) TCRγδ⁺ and TCRβ⁺ IEL from Gzmb CreERT2 × Rosa26 lsl-tdTomato mice, assessed 7 days after final tamoxifen or oil-only control administration. E) Quantification of tdTomato⁺ IEL across indicated time points, demonstrating the longevity of labelled IEL subsets. Data represent mean ± SEM from 4 mice per group. Statistical comparisons: ANOVA with Sidak’s post hoc correction (A, B). * p < 0.05, ** p < 0.01.

Article Snippet: Gzmb KI-Cre was generated by Ingenious Targeting Laboratory for Dr. Mahima Swamy.

Techniques: Injection, Expressing, Control